| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Impact FC Nixa LLC - 01 Impact FC Nixa - B2009 Ajax |
0S16-01CB09-002009 | Joshua Scott |
| A2 | Sporting Springfield - 01 Sporting Springfield - 09 Argentina |
0S02-01CB09-000025 | Marcos Soriano |
| A3 | Sporting Springfield - 01 Sporting Springfield - Bolivar 2009 |
0S02-01CB09-024682 | Thomas Bledsoe |
| A4 | Sporting Springfield - 01 Sporting Springfield- Branson Atletico Madrid 2009 |
0S02-01CB09-024680 | Cristian Mazilu |
| A5 | Southwest MO Rush SWMO Rush Cinza 2009B |
0S10-01CB09-000013 | Josephat Phiri |
| A6 | Southwest MO Rush Team Justice |
0S10-01CB09-000041 | Enver Medrano |
| A7 | United Springfield Academy - 01 USA 2009 Boys |
0S15-01CB09-000006 | Memo Latapi |
| A8 | Southwest MO Rush SWMO Rush Nero 2009B |
0S10-01CB09-010374 | Parviz Rakhmonov |
| A9 | Jaguar FC Jaguar FC 09 |
0S08-01CB09-000003 | Seth Cupp |